【摘要】 目的研究白藜芦醇对TRAIL诱导肝癌细胞SMMC-7721凋亡的影响及其机制。方法培养人肝癌细胞株SMMC-7721,分为对照组、TRAIL干预组、Res+TRAIL干预组,进行DNA末端原位标记(TUNEL)染色法作凋亡分析;Western Blot检测白藜芦醇对SMMC-7721细胞Survivn表达的影响。结果对照组SMMC-7721细胞的凋亡指数为(1.78±0.49)%,TRAIL干预组凋亡指数为(9.86±0.53)%,Res+TRAIL干预组中25μmol/L、50μmol/L、100μmol/L浓度Ros凋亡指数分别为(11.30±0.80)%,(20.89±0.69)%和(27.78±1.88)%。除Res+TRAIL干预组(25μmol/L)与TRAIL干预组比较无显著性差异(P>0.05),其余各组之间比较差异均有统计学意义(P均<0.05)。Western Blot测定结果显示:25μmol/L Ros处理组Survivin/β-actin的值与对照组相比无统计学差异(P>0.05);50μmol/L和100μmol/L Res处理组Survivin/β-actin的值较对照组显著降低(P<0.01);100μmol/L Res处理组Survivin/β-actin的值较50μmol/L显著降低(P<0.01)。结论 TRAIL对SMMC-7721细胞有凋亡诱导作用;Res对TRAIL诱导人肝癌细胞SMMC-7721凋亡有致敏作用,并且这种作用呈剂量依赖关系;其分子机制可能与下调凋亡抑制因子Survivin的表达有关。更多还原

【Abstract】 Objective It is to observe the influence of resveratrol on TRAIL-induced apoptosis in human hepatoma cell line SMMC-7721 and its mechanism. Methods SMMC-7721 cells were cultured in vitro and divided into three gropus: control group,TRAIL intervention group,resveratrol and TRAIL intervention group. After treatment with TRAIL / resveratrol and TRAIL for 24 hours,TUNEL staining were given to detect the effects of resveratrol on TRAIL-induced apoptosis in human hepatoma cell line SMMC-7721; Western blot technology was performed to detect the expression of apoptosis-related protein survivin in hepatoma apoptotic cells treated by resveratrol. Results The apoptotic rate of SMMC-7721 cells was( 1. 78 ±0. 49) % in control group,and was( 9. 86 ± 0. 53) % in TRAIL intervention group,was( 11. 30 ± 0. 80) %,( 20. 89 ±0. 69) %,( 27. 78 ± 1. 88) % respectively after cotreatment with 100 μg /L TRAIL and 25 μmol /L or 50 μmol /L or 100μmol/L resveratrol for 24 hours in resveratrol and TRAIL intervention group. There was significant difference between every two groups,excepted 25 μmol /L resveratrol intervention group with TRAIL intervention group( P < 0. 05). The results of detection with Western Blot technology indicated that after being treated by 50 μmol /L,100 μmol /L resveratrol for 24 hours,the value of surviving /β-actin was down-regulated significantly compared with control group( P < 0. 01); and there was significant difference between 50 μmol /L and 100 μmol /L resveratrol-treated group( P < 0. 01). Conclusion TRAIL can induce apoptosis of human hepatoma SMMC-7721 cells in vitro. Resveratrol can enhance TRAIL-induced apoptosis of SMMC-7721 cells in dose-dependent manner. This enhancement may be related to the inhibition of expression of survivin by resveratrol.更多还原