【摘要】 制备了辣根过氧化物酶/聚乙烯醇缩丁醛/碳纳米管修饰玻碳电极的过氧化氢生物传感器。以对苯二酚为电子媒介,采用电流时间法考察了该传感器对H2O2的催化性能,并将其与紫外分光光度法检测过氧化氢进行对比研究。结果表明,过氧化氢电化学传感器制备方法简便、操作简单、检测快速、线性范围宽、检出限低,而紫外分光光度法在重现性、稳定性及抗干扰性能上存在优势。更多还原

【Abstract】 A hydrogen peroxide biosensor has been made by horseradish peroxidase /polyvinyl butyral /multiwall carbon nanotube immobilizing on the glassy carbon electrode surface. Using hydroquinone as mediator,the electrocatalytic properties of this sensor toward hydrogen peroxide are observed by i-t curves,which are compared with ultraviolet spectrophotometry. The biosensor exhibits easy-making,simple operation,quick inspection,wider linear range and lower detection limit ability. In contrast,ultraviolet spectrophotometry has advantages in reproducibility,stability and noise immunity.更多还原