The DNA was extracted from mature Spinacia oleracea L.separately by the method of CTAB,then used as template after mixed by gender to be amplified with the optimized ISSR reaction condition.A total of 74 primers were tested with the same PCR cycling procedures.Only a female-associated fragment with a length of about 1 200 bp was generated with I62 primer.The I62 fragment was extracted from agarose gel and cloned into pUCm-T vector,and then transformed into E.coli JM109.It was sequenced after test.Results sh...
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