【摘要】 目的探讨红鲫(Cyprinus carpio)肥大细胞(MC)的不同鉴别方法。方法取已被迟缓爱德华菌感染的红鲫鳃组织,用Bouin氏液固定3 h,苏木精-伊红(HE)法、甲苯胺蓝(TB)法、阿利新蓝(AB)法、中性红法、Masson三色法、May-Grünwald Giemsa(MGG)法与免疫组织化学SABC法观察MC。同时使用Wright-Giemsa染色的头肾涂片法作为补充。结果阿利新蓝和甲苯胺蓝法显示肥大细胞良好,MGG法次之。免疫组织化学法检测类胰蛋白酶阳性肥大细胞数量较少,阳性反应弱。涂片法可将细胞分散开,并显示出不同发育阶段肥大细胞的特性。结论组织化学染色结合涂片法和免疫组织化学染色,可以更好的识别鱼类肥大细胞。更多还原

【Abstract】 Objective To compare different identifying methods of the mast cells of red crucian carp( Cyprinus carpio).Methods The gill filaments were taken from Cyprinus carpio infected with Edwardsiella tarda and fixed in Bouin’s solution.The hematoxylin-eosin( HE),toluidine blue( TB),Alcian blue( AB),neutral red,Masson trichrome,May-Grünwald Giemsa( MGG) stainings and streptavidin-biotin complex( SABC) immunohistochemical method were used to observe the mast cells. The smears of head kidney stained by Wright-Giemsa were used as a supplementary method. Results AB and TB were desirable staining solutions for clearly displaying mast cells,and MGG staining came second. Immunohistochemical method showed the small number of tryptase-positive mast cells and a weak positive reaction. The cells in smears were dispersed and showed different developmental stages. Conclusion The immunohistochemistry and the histochemical staining combined with Wright-Giemsa-stained smears are two good ways to identify the mast cells of fish.更多还原