【摘要】 目的建立一种快速、低成本的ABO血型基因分型方法。方法设计6对引物,建立扩增阻滞突变系统(ARMS),对30例中国汉族无关个体外周血样本的ABO基因型进行鉴定,并与血清学方法和直接测序结果进行比较。结果 ARMS技术检测30例样本结果同血清学方法、直接测序结果吻合率达到100%。结论该研究建立的ABO血型基因型鉴定方法可快速检测出28种ABO基因型,具有一定的临床应用价值。更多还原

【Abstract】 Objective To develop a rapid,cost-effective and reliable method for ABO genotyping. Methods Six pairs of primers were designed for the amplification refractory mutation system(ARMS). Thirty peripheral blood DNA samples,derived from Chinese Han unrelated individuals,were genotyped by ARMS. The results were compared with the corresponding data obtained from serological methods and DNA sequencing. Results The ABO genotyping results using ARMS were perfectly in line with the serological methods and DNA sequencing. Conclusion The method developed and optimized in this study is able to accurately and rapidly detect 28 kinds of ABO genotypes,indicative of a new approach applicable to clinical practice.更多还原