Isolation and identification of endophytic actinomycetes from the seeds of Camptotheca acuminata Decne.and isolation of antimicrobial substances from those endophytic actinomycetes
[Objective] The seeds of Camptotheca acuminata Decne.containing camptothecin(an anticancer agent),was investigated to explore the endophytic actinomycetes diversity and screen for new active compounds producing strain.[Methods] The seeds of C.acuminata Decne.were sliced and plated on different selective media after surface sterilization.Colonies that looked like actinomycetes were selected,and identified according to the colonial morphology and 16S rRNA sequence analysis.Strains isolated were tested for ant...
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