Objective To explore using dry powdered liver extract powder,trypticase,and peptone as main materials,it is the need for large-scale production,preparation was carried out for the medium production of tetanus toxin instead o f the traditional medium contained liver extracellular fluid,trypsin digestion of casein and concentrated peptone water. M ethods The prepared midea and the traditional media were both used in production process of tetanus toxin,from a simple procedure to a large scale gradually,differe...