【摘要】 目的:研究南通汉族群体17个常染色体STR基因座遗传多态性。方法:利用AGCU 17+1荧光检测试剂盒,对467例南通汉族无关个体的17个常染色体STR基因座(CSF1PO、D13S317、D16S539、D18S51、D19S433、D21S11、D2S1338、D3S1358、D5S818、D6S1043、D7S820、D8S1179、FGA、Penta E、TH01、TPOX、vWA)进行扩增,用ABI 3100xl遗传分析仪和GeneMapper ID v3.2软件进行STR分型分析,用Cervus 3.0软件进行等位基因频率和遗传学参数统计分析,并检验Hardy-Weinberg。结果:南通汉族群体中,17个STR基因座基因频率(GF)在0.001 1~0.509 6之间,杂合度(H)在0.636 0~0.933 6之间,期望杂合度(He)在0.626 8~0.921 9,个体识别能力(DP)在0.797 4~0.986 5之间,非父排除率(PE)在0.3363~0.8645之间,多态性息含量(PIC)在0.563 5~0.915 4之间,结果经χ2检验均符合Hardy-Weinberg平衡定律(P>0.05)。结论:南通汉族群体17个STR基因座具有较高多态性,能够满足南通汉族人群个体识别和亲权鉴定的需要,也可在群体遗传学等相关研究和实践中有较高的价值。更多还原

【Abstract】 Objective: To investigate the genetic polymorphism of 17 short tandem repeat( STR) loci in autosomes of Han population living in Nantong area. Methods: AGCU17 + 1 kit was used to amplify the 17 STR loci( CSF1 PO,D13S317,D16S539,D18S51,D19S433,D21S11,D2S1338,D3S1358,D5S818,D6S1043,D7S820,D8S1179,FGA,Penta E,TH01,TPOX,vWA) from 467 unrelated individuals of Han population in Nantong area,and ABI310 xl analyzer and software GeneMapper ID v3. 2 were used for STR genotyping. The allele frequencies and genetic parameters were calculated with software Cervus 3. 0 and tested by Hardy-Weinberg equilibrium. Results: The genetic frequencies in 17 STR loci in Han population of Nantong area ranged from 0. 001 1 to 0. 509 6,and the heterozygosities( H) were from 0. 6360 to 0. 9336,the expected heterozygosity( He) between 0. 626 8 and 0. 921 9. The discrimination powers( DP) varied from 0. 797 4 to 0. 986 5,the probabilities of exclusion( PE) from 0. 336 3 to 0. 864 5 and the polymorphism information contents( PIC) from 0. 563 5 to 0. 915 4. These results were complied with Hardy-Weinberg equilibrium( P > 0. 05). Conclusion: The 17 STR loci are highly polymorphic in the Han population living in Nantong area. Our findings can meet the need for individual identification and paternity testing,and may apply to genetic research.更多还原