【摘要】 利用Minitab软件对甘蔗渣酶解工艺条件进行优化研究。采用Plackett-Burman设计,从温度、pH值、加酶量、液固比和转速5个影响因素中,筛选出具有显著效应的温度、pH值和加酶量3个主要影响因素。然后采用响应面法对3个主要影响因素做进一步优化研究。优化结果表明:温度47.6℃,p H=4.76和加酶量37.76 Fpu/g时,综纤维素转化率可达74.5%。该条件下,实际试验值为74.44%,与预测值吻合较好。更多还原

【Abstract】 The Minitab software was adopted to optimize enzymatic hydrolysis process of sugarcane bagasse. Firstly,Plackett-Burman design was used to evaluate five variables including temperature, p H value, cellulase adding dosage,ratio of liquid and solid, agitation speed. The significant impact factors were screened as temperature, p H value andcellulase adding dosage. Then the response surface method(RSM) was used for further optimization study. The resultsshowed that the optimum conditions are temperature of 47.6 ℃, pH of 4.76, cellulase adding dosage of 37.76 Fpu/g, theholocellulose conversion rate can attain to 74.5%. Under these conditions, the actual concentration of the holocelluloseconversion rate is 74.44%, is in good agreement with the predicted values.更多还原