【摘要】 目的是检测运动前后大鼠血液及各组织H2S含量以及各个组织生成酶活性,分析运动后各组织H2S含量及其生成酶活性的变化特点。以雄性SD大鼠为实验对象,采用敏感硫电极法检测大鼠心脏,肾脏,肺,肝脏,骨骼肌,脑等组织H2S含量,对结果进行相关性分析。雄性SD大鼠16只,随机分为2组:对照组、运动组。运动组大鼠做连续1小时的一次大强度性负重(10%体重)游泳运动,运动后即刻对大鼠取心脏,肾脏,肺,肝脏,脑组织、腓肠肌、腹主动脉血进行分析,采用敏感硫电极法测定各组织的H2S含量及其生成酶活性;对照组大鼠直接取样测定。经运动后与对照组相比除大鼠脑组织H2S含量明显降低外,运动组大鼠各组织系统H2S含量较对照组有大幅度升高。经运动后与对照组相比除大鼠脑组织H2S生成酶活性略有降低外,运动组大鼠各组织H2S生成酶活性较对照组均有不同程度的升高,尤以心脏、肺脏组织有大幅度升高。敏感硫电极法测量范围广,敏感性强,稳定性和重复性均良好,可应用于大鼠脑、心脏、肾脏、肝脏、肺、骨骼肌H2S测定的研究,但在测试血浆H2S含量时精确度略显不够。经一次性大强度运动后,与对照组相比大鼠心脏,肺脏,肝脏,肾脏,骨骼肌器官的H2S/生成酶体系均有不同程度的提升。运动使内源性一氧化氮升高从而造成H2S/生成酶体系的升高。一次性大强度运动可增加各个组织无氧代谢酶活性,推测一次性大强度运动使机体无氧代谢酶活性的升高与H2S/生成酶系统活性的上升有着必然联系。大鼠运动后脑组织H2S/生成酶系统活性降低的原因还不是十分清楚,推测测定时大鼠脑组织中的硫化氢生成酶胱硫醚—β—合酶(CBS)还未合成。大鼠血浆硫化氢浓度变化趋势同大鼠其它组织,验证了血管平滑肌CSE在大鼠运动后的表达同其它各组织。更多还原

【Abstract】 [Purpose]:This study aims to rats before and after the determination of hydrogen sulfide found in various tissues and organs in vivo determination of sulfide The hydrogen content appropriate;After training in detection of hydrogen sulfide content in the blood and various organizations and various organizations generating activity.H2 S content of the training,organization and analysis of activity generated the change.[Methods] :Male Sprague-Dawley rats for experiments by using sulfur electrode sensitive detection rat heart,kidney,lung,H2 S content of the liver and other organizations,the results are analyzed and compared.Sixteen male Sprague-Dawley rats were randomly divided into two groups :control group,campaign group.Rats to do for a one-hour high-intensity load(10% weight) swimming.The heart was all the rats immediately after sports,kidney,lung,liver,brain,muscle,abdominal aortic blood analysis using sensitive electrodes measured the sulfur and hydrogen sulfide content generating activity.[Results] :Compared with the control group campaigned rat brain tissue in addition to hydrogen sulfide levels decreased significantly,H2 S content of the campaign group of the organizations are significantly higher than the control group.Compared with the control group except campaigned rat brain activity generated H2 S slightly lower,campaigning group generated H2 S activity of the organizations have varying degrees of increase compared with the control group.especially in the heart and lungs are significantly increased.[Conclusions] :Sensitive to sulfur electrode wide measurement range,sensitivity,stability and reproducibility are good and can be used in rat brain,heart,kidney,liver,lung,muscle Determination of hydrogen sulfide.However,in testing at the plasma of hydrogen sulfide content is slightly insufficient precision.After one-time high-intensity exercise,compared with the control group of rat heart,lung,liver,kidney,H2 S skeletal organ / enzyme systems have different levels of upgrading.Speculate as to enable movement of endogenous nitric oxide results in increased hydrogen sulfide / enzyme system increased.As a one-time high-intensity exercise can increase various organizations anaerobic metabolism activity,speculate one-time high-intensity anaerobic exercise so that the body’s metabolic activity increased with the hydrogen sulfide / enzyme system activity is the rise necessarily related.Rat brain tissue H2 S / weather enzyme system activity is the reason or not is not very clear,it is speculated in rat brain tissue formation of hydrogen sulfide CBS enzyme synthesis yet.Change in plasma of hydrogen sulfide rats is similar with the trend of other organizations,certificating that the expression of vascular smooth muscle CSE movement in rats is similar to that of other organizations.更多还原