【摘要】 以SGC7901和SGC7901/ADR为细胞模型,检测了冬凌草活性部位与化疗药物联用后,对SGC7901/ADR耐药性的逆转效应;冬凌草活性部位处理细胞后,检测耐药细胞内阿霉素的蓄积变化、耐药细胞P-糖蛋白(P-gp)的表达水平以及mdr1基因的表达变化。结果显示,冬凌草氯仿部位和乙酸乙酯部位可以有效提高化疗药物阿霉素在SGC7901/ADR细胞内的蓄积,降低P-gp的表达,降低mdr1基因的转录。冬凌草逆转胃癌耐药细胞SGC7901/ADR多药耐药性的活性部位是冬凌草氯仿部位和乙酸乙酯部位,其逆转作用与抑制P-gp的表达相关。更多还原

【Abstract】 The effect of Rabdosia rubescens on the expression and function of P-glycoprotein( P-gp) in SGC7901 /ADR cell was investigated in this study. The potential bio-active extract fractions were selected to carry out experiments in SGC7901 /ADR cells. After treatment with chloroform fraction and ethyl acetate fraction of R. rubescens,the cell viability was examined by MTT assay,the expression of P-gp in SGC7901 /ADR and SGC7901 cells was determined by fluorescent-labeled antibody technique,and the expression of mdr1 gene was detected by RT-PCR. The experimental results indicated that chloroform fraction and ethyl acetate fraction of R. rubescens increased the intracellular accumulation of adriamycin in SGC7901 /ADR cells,and decreased the expression of mdr1 gene and P-gp. Therefore,chloroform fraction and ethyl acetate fraction of R. rubescens had effects on reversal of multidrug resistance of SGC7901 /ADR cells,and the mechanism was possibly associated with the inhibition of the expression of P-gp.更多还原