【摘要】 建立采用UHPLC同时测定野菊花中绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸、4,5-二咖啡酰奎尼酸和蒙花苷含量的分析方法。UHPLC分析条件:Agilent ZORBAX RH C18色谱柱(50 mm×2.1 mm,1.8μm),检测波长326 nm,流动相为乙腈-0.1%磷酸水进行梯度洗脱,流速0.4 mL/min,柱温25℃。实验结果显示绿原酸、3,4-二咖啡酰奎尼酸、3,5-二咖啡酰奎尼酸、4,5-二咖啡酰奎尼酸、蒙花苷分别在1.20~24.00、1.16~23.20、2.23~44.60、1.75~35.00、2.25~45.00μg/mL范围内线性关系良好(r>0.9998),平均回收率分别为98.08%、98.42%、97.75%、98.27%、98.36%;RSD分别为0.38%、1.50%、0.77%、0.81%、0.62%。UHPLC法分析速度快、高效、重复性好、结果准确简便,可用于野菊花中四种有机酸和蒙花苷含量的测定。更多还原

【Abstract】 The objective of this study was to develop a UHPLC method for the simultaneous determination of five active components,namely chlorogenic acid,3,4-O-dicaffeoylquinic acid,3,5-odicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid and buddleoside in Flos Chrysanthemi Indici. The chromatographic separation was performed on a Agilent ZORBAX RH C18( 50 mm × 2.1 mm,1.8 μm) column with acetonitrile-0.1% phosphoric buffer as mobile phases. The flow rate was 0. 4 mL /min. The DAD detection wavelength was set at 326 nm and the column oven temperature was maintained at25 ℃. Chlorogenic acid,3,4-O-dicaffeoylquinic acid,3,5-O-dicaffeoylquinic acid,4,5-O-dicaffeoylquinic acid and buddleoside showed good linearity( r > 0. 9998) within the range of 1. 20-24. 00,1. 16-23. 20,2. 23-44. 60,1. 75-35. 00,2.25-45. 00 μg /mL,respectively; their respective average recoveries were 98. 08%( RSD = 0. 38%),98. 42%( RSD = 1.50%),97. 75%( RSD = 0. 77%),98. 27%( RSD = 0. 81%),98. 36%( RSD = 0. 62%). Based on these results,it was concluded that the developed UHPLC method was simple,sensitive,accurate and reproducible. It can be used for the quality control of the five active components of Flos Chrysanthemi Indici.更多还原