【摘要】 本实验目的在于探讨海带多糖对实验性高脂血症小鼠瘦素及其受体水平的影响及其可能的机制。将健康雄性昆明小鼠80只,随机分为正常饲料组和高脂饲料组,后组以高脂饲料喂养建立高脂血症动物模型。将建模成功的小鼠随机均衡分为高脂模型组和海带多糖治疗组,后组以海带多糖灌胃干预。自动生化分析仪检测小鼠空腹血脂水平,ELISA测定血清瘦素(Leptin)含量,免疫组织化学法和Western blot检测下丘脑组织中瘦素受体(Lep-R,OBR)蛋白表达,RT-PCR检测OBR mRNA表达。结果示海带多糖治疗组小鼠血脂及血清Leptin水平较模型组显著降低(P<0.05),下丘脑OBR mRNA及其蛋白表达水平较模型组显著升高(P<0.05)。说明海带多糖可以通过提高下丘脑OBR的水平,改善瘦素抵抗,从而发挥降脂作用。更多还原

【Abstract】 This experiment was designed to study the effects and possible mechanism of Laminaria japonica polysaccharide(LJPS) on leptin level in serum and leptin receptor expression in hypothalamus of hyperlipemia mice. 80 male mice were randomly divided into control group,model group,and LJPS treatment groups. The hyperlipemia model of mice were established by feeding fat-rich forage for 4 weeks and treated by LJPS orally. Lipids levels in serum were determined by automatic biochemical analyzer and leptin levels in serum were determined by enzyme linked immunosorbent assay(ELISA). The immunohistochemistry and western blot were used to detect the expressions of leptin receptors(OBR)proteins and RT-PCR was used to detect the expression of OBR mRNA in hypothalamus tissue. After treated with LJPS,the serum levels of lipids and leptin decreased significantly compared with those in model group( P < 0. 05). In hypothalamus tissue,the expression levels of OBR mRNA and its protein were significantly higher than those in model group(P <0.05). Hence,it was concluded that LJPS might reduce blood lipid level in hyperlipemia mice by promoting the expressions of OBR and reducing leptin resistance.更多还原