【摘要】 对森林土磷脂脂肪酸超声提取工艺进行了研究。在单因素试验的基础上,选择超声温度、超声时间、提取剂用量为自变量,以森林土磷脂脂肪酸的提取率为响应值,进行Box-Behnken中心组合实验设计,采用响应面法(RSM)评估了这些因素对森林土磷脂脂肪酸提取率的影响。结果表明:在超声温度20℃,超声时间30 min,提取剂用量21 mL/8 g土壤条件下,磷脂脂肪酸最大提取率达到45.57 nmol/g(n=3,单体PLFAs个数35个)。表明响应面法超声辅助提取优化磷脂脂肪酸工艺的研究方法简便、可靠。更多还原

【Abstract】 The objective of this study was to optimize ultrasonic-assisted extraction of phospholipid fatty acids(PLFAs)from forest soil using response surface methodology(RSM). Based on single factor experiments,extraction temperature,time and amount of extraction solvent were selected for Box-Behnken central composite designing. RSM was employed to study the effect of these factors on the yield of PLFAs. The results indicated that the optimal extraction conditions were20 ℃ as extraction temperature,21 mL as amount of extraction solvent,with 30 min of extraction. Under these conditions,the maximal extraction yield reached 45. 57 nmol /g(n = 3). The response surface methodology used for the optimization of ultrasonic-assisted extraction conditions of PLFAs from forest soil was simple and reliable.更多还原