【摘要】 利用高效液相色谱法,分别检测并对比红花药材中羟基红花黄色素A和金橙Ⅱ的浓度,从而建立对比筛选红花样品中金橙Ⅱ的方法。结果在46个红花样品中,推测有26个可疑阳性样品;而通过对比筛选法能将可疑阳性样品数减至2个。经HPLC-MS法确证了对比筛选法的结果。检测羟基红花黄色素A(HSYA)的色谱条件为:甲醇-乙腈-0.01%磷酸溶液(体积比为26∶2∶72)为流动相。检测金橙Ⅱ的色谱条件为:乙腈-0.025 mol/L溶液(40∶60)为流动相。此法相比于常规只检测红花中金橙Ⅱ的方法,筛选效率与精确度更高,并且更经济、适应性更广。更多还原

【Abstract】 In this study,a HPLC method for contrastively screening of orange-II in safflower samples was established by comparing concentrations of HSYA and orange II. For hydroxysafflor yellow A( HYSA) chromatographic testing condition,methanol-acetonitrile-0. 01 mol / L phosphoric Acid( 26∶ 2∶ 72) was used as mobile phase. For Orange II chromatographic testing condition,acetonitrile-0. 025 mol / L monopotassium phosphate( 40∶ 60) was used as mobile phase. After testing the concentration of orange-II of all 46 safflower samples,26 were indicated as suspicious positive samples. However,the number of suspicious positive samples was reduced to 2 using contrast screening method. The screening results were ensured with LC / MS method. Hence,the results showed that the efficiency of contrast screening method was superior to conventional orange-II screening method.更多还原