【摘要】 目的探讨CABG术前单次他汀负荷对心肌特异mi RNA(mi RNA-1、mi RNA-133、mi RNA-208、mi RNA-499)表达的影响。方法 CABG患者术前12h随机分为两组:A组常规服用20 mg阿托伐他汀,B组术前单次服用80 mg阿托伐他汀。术中随机获取右心耳组织,A组5例,B组3例,健康人右心耳组织4例作为C组,荧光定量聚合酶链式反应检测心脏特异性mi RNA的表达量(mi RNA-1,mi RNA-133,mi RNA-208和mi RNA-499)。结果 mi RNA-1,mi RNA-133a,mi RNA-133b,mi RNA-208a,mi RNA-208b,mi RNA-499a-3p,mi RNA-499a-5p,和mi RNA-499b-3p在三组中的表达无差异。与A组和C组相比,mi RNA-499b-5p在B组中明显高表达。结论 CABG术前单次大剂量他汀负荷的心肌保护作用,其机制之一可能通过上调mi RNA-499b-5p实现。更多还原

【Abstract】 Objective To explore the cardiac protection effects of a single high loading dose of statins therapy before coronary artery bypass grafting(CABG)on cardiac specific mi RNAs(mi RNA-1,mi RNA-133 mi RNA-208 and mi RNA- 499). Methods Patients undergoing CABG were divided into two groups randomly 12 hours before cardiac operation:Group A,receiving standard- dose of 20 mg atorvastatin,Group B,receiving single- dose of 80 mg atorvastatin. 5 right atriums from group A and 3 from group B were achieved during the operation. Accompanied with 4 right atriums in healthy population as group C,the gene expression on cardiac specific mi RNAs(mi RNA- 1,mi RNA- 133,mi RNA- 208 and mi RNA- 499)of the three groups were examined by a stem- loop quantitative reverse transcriptasepolymerase chain reaction(q RT- PCR). Results The expressions of cardiac specific mi RNAs including mi RNA- 1,mi RNA-133 a,mi RNA-133 b,mi RNA-208 a,mi RNA-208 b,mi RNA-499a-3p,mi RNA-499a-5p and mi RNA-499b-3p in group A,group B and C were not significant changed. Strikingly,mi RNA-499b-5p was significantly increased in group B comparing to both healthy group C(P=0.005)and group A(P=0.010). Conclusions The cardiac protection effect of a single high loading dose of statins therapy before CABG maybe relates to elevated mi RNA-499b-5p.更多还原