【摘要】 目的:建立高效液相色谱(HPLC)法测定凉血消风汤中绿原酸含量。方法:采用外标法。Hypersil ODS2(250mm×4.6 mm,5μm)色谱柱为固定相,乙腈-0.4%磷酸溶液(13∶87)为流动相,检测波长为327 nm,流速为1.0 ml/min。结果:线性范围为4.0~40.0μg/ml(r=0.999 1),平均加样回收率为101.0%,RSD为1.9%(n=6)。结论:本方法专属性强,精密度和稳定性良好,结果准确,可用于凉血消风汤的质量控制方法。更多还原

【Abstract】 Objective:To establish an HPLC method for determination of chlorogenic acid in Liangxue Xiaofeng Tang. Methods: An external method with Hypersil ODS2(250 mm × 4. 6 mm,5 μm) column as fixed phase and acetonitrile-0. 4% phosphoric acid(13∶ 87) as mobile phase was adopted. The detection wave length was 327 nm. The flow rate was 1. 0 ml / min. Results:The linearity range was 4. 0 ~ 40 μg / ml( r = 0. 999 1),The average recovery was 101. 0%,RSD was 1. 9%( n = 6). Conclusion:The method is simple,rapid and reliable. So it can be used as quantitativc determination for quality control of Liangxue Xiaofeng Tang.更多还原