【摘要】 分别利用分光光度法和1-苯基-3-甲基-5-吡唑啉酮(PMP)衍生化/HPLC法分析了12批不同批次药材的决明子多糖及其水解产物中的单糖组分,以水解的单糖组分建立了决明子多糖的HPLC指纹图谱,并研究了决明子多糖的组成特征。结果表明,经过纯化的决明子多糖含量在93.41%~98.57%之间,以甘露糖为参照峰建立了12批药材的指纹图谱,相似度在0.963~0.999之间,多糖水解产物中单糖组分的含量在1.617~304.5 mg/g之间,7个共有峰组分含量大小分布均依次为:甘露糖>木糖>半乳糖>葡萄糖>阿拉伯糖>葡萄糖醛酸>氨基半乳糖,显示了决明子多糖的基本组成结构特征,12批药材的决明子多糖中各单糖的平均摩尔比为甘露糖∶木糖∶半乳糖∶葡萄糖∶阿拉伯糖∶葡萄糖醛酸∶氨基半乳糖=43.0∶24.2∶15.4∶5.6∶5.5∶3.4∶2.9。指纹图谱相似度分析和多糖的组成特征分析对市售药材的分析鉴定结果一致,可作为决明子多糖结构分析及其质量控制的依据。更多还原

【Abstract】 Analysis of polysaccharides and their hydrolyzed products in Cassia obtusifolia L. was performed by UV spectrometry and high performance liquid chromatography( HPLC),respectively. The composition characteristics of polysaccharides were developed,including 12 batches of Cassia obtusifolia L. The results showed that the content of polysaccharides extracted from samples were between93. 41% and 98. 57% after purification. Under the optimized hydrolysis conditions, monosaccharides obtained were determined by HPLC with 1-phenyl-3-methyl-5-pyrazolone( PMP) derivatization.Based on HPLC data of 12 batches of samples,fingerprints of polysaccharides in Cassia obtusifolia L.were established. Common characteristic peaks were identified as mannose,glucuronic acid,galactosamine,glucose,galactose,xylose and arabinose,and their content was in the range of 1. 617-304. 5 mg / g. Polysaccharides for 12 batches of Cassia obtusifolia L. basically consisted of seven monosaccharides,named mannose, xylose, galactose, glucose, arabinose, glucuronic acid and galactosamine with an average molar ratio of 43. 0 ∶ 24. 2 ∶ 15. 4 ∶ 5. 6 ∶ 5. 5 ∶ 3. 4 ∶ 2. 9. Fingerprints and molar ratio methods were successfully applied in the analysis of medicinal materials sold in market.更多还原