【摘要】 制备了纳米银/壳聚糖(AgCS)与乙炔黑(AB)复合修饰电极(AgCS-AB/GCE),用紫外可见分光光度法、扫描电镜法对AgCS进行表征,用交流阻抗法对不同电极进行表征,用循环伏安法(CV)研究了乙萘酚(β-N)在AgCS-AB/GCE上的循环伏安行为。结果表明,在pH 6.5的PBS缓冲液中,β-N在该修饰电极上出现一明显氧化峰,在60~240 mV/s扫速范围内,β-N的氧化峰电流(I pa)与对应扫速平方根(v1/2)呈良好的线性关系,表明β-N在该电极上的电化学过程为受扩散控制的不可逆过程。计算了电极过程的动力学参数,运用方波伏安法测定不同浓度β-N的方波伏安曲线,结果表明β-N的氧化峰电流与其浓度在3.0×10-7~2.0×10-4mol/L范围内呈良好线性关系(r=0.996),检出限(S/N=3)为1.24×10-7mol/L,加标回收率为99%~105%。更多还原

【Abstract】 A new biosensor was fabricated with sol of nanosilver/chitosan (sol-AgCS) and acetylene black (AB) to form a compound film on a glassy carbon (GCE) electrode. The AgCS was characterized by UV spectrometry and scanning electron microscopy (SEM). The different GCEs were characterized by electrochemical impedance spectroscopy (EIS). The electrochemical behaviors of ethyl naphthol (β-N) on AgCS-AB/GCE were investigated by cyclic voltammetry (CV). The results showed that β-N exhibited a sensitive oxidation peak in pH 6. 5 PBS buffer solution. The oxidation peak current (I pa) was linear to v1/2in the range of 60-240 mV/s,which suggested that the electrode process of β-N was controlled by diffusion. The reaction electronic numbers,diffusion coefficient and other important parameters of β-N were calculated. The different concentrations of β-N were determined by square wave voltammetry,and a good linearity between peak current and concentration of β-N was observed in the range of 3. 0 × 10-7-2. 0 × 10-4mol/L. The detection limit (S/N = 3) was 1. 24 × 10-7mol/L and the spiked recoveries were in the range of 99%-105%.更多还原