【摘要】 目的:验证心房钠尿肽(ANP)在高血压心力衰竭发展过程中的动态变化和氨氯地平保护心肌细胞的作用。方法:将雄性SD大鼠行腹主动脉结扎术,术后第4周随机选择40只大鼠分为高血压模型对照组(模型组)和苯磺酸氨氯地平低剂量组、中剂量组、高剂量组。另10只健康雄性SD大鼠作为假手术组。灌胃给药,模型组与假手术组给予生理盐水。结果:12周后,ANP水平模型组较假手术组升高,高剂量组较模型组降低(P<0.05);左心室收缩压及左室内压力最大上升速率模型组较假手术组降低,高剂量组较模型组升高(P<0.05);左心室舒张末期压力及左室内压力最大下降速率高、中、低3组之间比较差异有显著性(P<0.05);全心质量指数及左室质量指数模型组较假手术组降低,高剂量组较模型组升高(P<0.05);高剂量组心肌坏死程度与纤维化程度均较模型组减轻(P<0.01)。结论:ANP随心衰加重逐渐上升。氨氯地平可抑制心肌重构,并在一定范围内与药物剂量相关,这种影响是通过干预ANP的分泌实现的。更多还原

【Abstract】 Objective To verify the dynamic changes of ANP during the diastolic heart failure and the protective effect of amlodipine. Methods Male SD rats underwent the abdominal aorta ligation. Four weeks after Surgery, 40 rats were divided into 4 groups: hypertension model group(group B), low-dose group(group C),mid-dose group(group D), high-dose group(group E). Another 10 healthy male SD rats were used as sham group(group A). During the experiment, different drugs were gavaged to different groups and the normal saline was used for control group. Results After 12 weeks, ANP levels increased in group B much more than in group A, while lower in group C(P < 0.05); LVSP and dp / dt max decreased in group B significantly than in group A,while higher in group C than in group B(P < 0.05); LVEDP and-dp / dt max had no statistically significant(P >0.05); HW / BW and LW / BW decreased in group B than in group A, while higher in group C than in group B(P < 0.05); The extent of myocardial necrosis and fibrosis in group C were much lower than in group B. But there was no Significant differences(P > 0.05). Conclusion ANP level gradually increased accompanying with the aggravation of heart failure, which is related with the dose of the drug within limits. Amlodipine can inhibit cardiomyocyte remodeling by interfering ANP secretion.更多还原