【摘要】 目的:本研究旨在探索Th17细胞分泌的标志性细胞因子IL-17在口腔扁平苔藓(OLP)局部病变中的作用机制。方法:24例网状型OLP患者、19例萎缩糜烂型OLP患者和13位健康志愿者被募集参与本实验。采用实时定量PCR实验检测IL-17 mRNA和CCL20 mRNA在OLP组织中的表达情况。结果:网状型OLP组和萎缩糜烂型OLP组口腔黏膜病损组织中IL-17基因的表达显著高于正常口腔黏膜组织(P=0.009 5,P<0.000 1),同时其在萎缩糜烂型OLP组组织中的表达量明显高于网状型OLP组(P=0.001 2)。CCL20基因在网状型OLP组和萎缩糜烂型OLP组局部组织中的表达显著高于对照组(P=0.035 7,P<0.000 1);在萎缩糜烂型OLP患者局部组织中的表达较网状型OLP组升高明显。而且OLP患者病损组织中CCL20 mRNA的表达随IL-17 mRNA的表达升高而增加,IL-17与病损局部的CCL20的产生呈正相关(P=0.003)。结论:IL-17可诱导角化细胞释放趋化因子CCL20,这一信号通路可能是促进相关炎症细胞向OLP局部病损组织迁徙和浸润的重要途径。更多还原

【Abstract】 Objective To investigate the mechanism of IL-17, the signature cytokine produced by Th17 cells, in OLP lesion. Methods 24 patients with reticular OLP, 19 patients with atrophic-erosive OLP and 13 healthy volunteers were enrolled in this study. Real-time quantitative PCR(real-time qPCR) was performed to analyze the expressions of the production of IL-17 and CCL20 mRNA. Results The expressions of IL-17 mRNA in reticular OLP and atrophic-erosive OLP were significant higher than that in healthy oral mucosa(P = 0.0095, P <0.0001, respectively), meanwhile, remarkable increased IL-17 expression in atrophic-erosive OLP group was found compared with reticular OLP group(P = 0.0012). Additionally, the expressions of CCL20 mRNA in reticular OLP and atrophic-erosive OLP were significant higher than that in control group(P = 0.0357, P < 0.0001, respectively),meanwhile, CCL20 expression in atrophic-erosive OLP was higher than that in reticular OLP. The expressions of CCL20 mRNA rises with the increased expression of IL-17, and were positive correlated with IL-17 expressions in OLP lesions(P = 0.003). Conclusions IL-17 production can induce chemokine CCL20 expression in OLP lesion.The signal pathway may promote the migration and infiltration of inflammatory cells in OLP lesions.更多还原