【摘要】 目的:探讨HU-308缓释涂层对大鼠成骨细胞(ROBs)生物学特性的影响。方法:利用层层自组装技术在经碱热处理后的钛片表面制备含不同浓度HU-308的肝素/壳聚糖缓释涂层。以碱热处理的钛片为对照组(C组),肝素/壳聚糖涂层组(P组)及含不同浓度HU-308的肝素/壳聚糖涂层组(T组:T1、T2、T3、T4组)为实验组,在不同时间点,检测ROBs在涂层表面的黏附、增殖以及ALP活性。结果:T4组涂层(HU-308浸渍浓度为0.025 mg/L)表面ROBs的黏附、增殖以及ALP活性均强于P组及C组。结论:低浓度HU-308缓释涂层可提高ROBs在其表面的黏附、增殖和ALP活性。更多还原

【Abstract】 Objective: To observe the the effects of sustained-release coatings of HU-308 on the biological characteristics of rat osteoblasts( ROBs) on Ti surface. Methods: The heparin /chitosan sustained release coatings containing HU-308 at different concentrations were prepared by layer-by-layer assembly technology on the alkali-and heat-treated Ti surface. The alkali-and heat-treated Ti samples were randomly divided into six groups,the samples in C group were used as control,in P group were treated by heparin /chitosan coatings and in T1,T2,T3 and T4 groups were treated by heparin /chitosan sustained-release coating containing HU-308 at different concentrations. The adhesion,proliferation and ALP activity of ROBs cultured on the coatings were assessed at different times.Results: The adhesion,proliferation and ALP activity of ROBs of T4 group( the impregnating concentration of HU-308 was 0. 025 mg /L) were the highest. Conclusion: The sustained-release coating with low concentration of HU-308 may improve the adhesion,proliferation and ALP activity of ROBs.更多还原