【摘要】 目的:研究腺苷脱氨酶(ADA)和甲氨蝶呤(MTX)对破骨细胞形成的作用。方法:用大鼠的全骨髓细胞培养体系(WBMCs)来评价破骨细胞形成。用半定量RT-PCR来评估MTX对ADA mRNA水平的影响。结果:全骨髓细胞培养系中,只有腺苷和脱氧腺苷消除了MTX对破骨细胞形成的抑制作用;MTX可抑制ADA mRNA的表达。结论:MTX抑制ADA表达,ADA表达下降使腺苷和脱氧腺苷表达升高,使MTX对破骨细胞的抑制作用减弱。更多还原

【Abstract】 Objective: To study the effects of adenosine deaminase and methotrexate(MTX) on osteoclastogenesis. Methods: The culture system of rat whole bone marrow cells(WBMCs) was utilized to evaluate osteoclastogenesis. Semi-quantitative RT-PCR was used to examine the expression of ADA mRNA in WBMCs culture system. Results: In the WBMCs culture system for evaluating osteoclastogenesis,MTX-induced suppression of osteoclastogenesis was abrogated by adenosine or deoxyadenosine. The expression of ADA mRNA was markedly suppressed by MTX treatment. Conclusion: ADA decreased the MTX-induced suppression of osteoclastogenesis because MTX inhibits ADA expression followed by the increase of adenosine and deoxyadenosine.更多还原