【摘要】 建立了测定腐竹中乌洛托品的方法。以乙腈提取腐竹中乌洛托品,加盐酸水解生成甲醛,以3-甲基-2-苯并噻唑啉酮腙盐酸盐水合物(酚试剂)和硫酸高铁铵与甲醛反应生成蓝绿色化合物,并用中性氧化铝吸附除脂后,在630nm测定吸光度间接计算乌洛托品含量。实验考察了盐酸用量、水解温度、吸附剂种类、吸附条件、反应时间等条件的影响。实验结果显示,脂肪、糖、蛋白质、苯丙氨酸等对测定结果没有干扰。亚硫酸盐对其有轻微干扰,但不影响定性检测。乌洛托品溶液在0.6~6.0μg/mL范围内线性关系良好。腐竹样品加标测定的平均回收率为88.2%。检出灵敏度好,可用于腐竹中乌洛托品的快速检测。更多还原

【Abstract】 The method for determing methenamine in yuba was set up.The sample was extracted by acetonitrile.Methenamine was hydrolyzed into formaldehyde by hydrochloric acid.The formaldehyde can be reacted with 3- methyl-2-benzothiazolinone ketone hydrazone hydrochloride(phenol reagent) and ferric ammonium sulfate to form a blue- green compound,which can be measured by spectrophotometry at630 nm after degreasing by neutral alumina.Some effect factors of experimental conditions were investigated like the amount of hydrochloric acid,hydrolysis temperature,adsorbent category and adsorption condition,reaction time and so on.The result showed that the determination was not interfered by fat,sugar,protein and phenylalanine,while slightly interfered by sulfite,which did not affect the determination conclusion.The method showed a good linearity at the range of 0.6~6.0μg/mL of methenamine.The average recovery was 88.2%.The method is sensitive,and can be used to detect methenamine in yuba.更多还原