【摘要】 目的 定量分析双源CT灌注扫描各参数在索拉非尼靶向治疗大鼠肝癌模型前后的变化特点,评估各灌注参数与MVD及VEGF之间的相关性的研究。方法 荷瘤大鼠34只,随机分为实验组(26只),对照组(8只)。分别给予索拉非尼及含等剂量溶剂的生理盐水20 mg/d,共灌胃21 d。治疗前后应用双源CT分别进行灌注扫描,观察2次灌注参数及肿瘤最大径的变化,第2次扫描结束后行HE及免疫组化检查。结果 (1)实验组大鼠治疗前后肝癌区BF、BV、pBV、ALP有统计学差异(P值均<0.05),而MPI、PVP、HPI等值均无统计学差异(P值均≥0.05)。对照组治疗前后肿瘤区域所有灌注参数均无统计学意义。(2)实验组治疗前后肿瘤最大径平均约为(0.828±0.319) cm和(0.573±0.435) cm (P=0.000),对照组肿瘤治疗前后最大径平均约为(0.816±0.401) cm和(1.370±0.332) cm(P=0.005)。(3)MIP、ALP分别与VEGF及MVD呈正相关。结论 双源CT灌注扫描能够作为一项非侵袭性替代参数来评估经索拉非尼治疗后大鼠种植性肝癌的血流动力学变化。更多还原

【Abstract】 Objective To analyze the changes of perfusion parameters of dual-source CT before and after treatment of liver cancer in a rat model with sorafenib quantitatively.Methods 34 tumor-bearing rats were randomly divided into an experimental group(n=26) and a control one(n=8).Intragastric administration has been performed with sorafenib at a dose of 20 mg/d in experimental group for 21 days,so was the saline in the control one at the same dose.The dual-source CT perfusion scan was also performed before and after the treatment,and the perfusion parameters and tumor diameters were recorded and measured.Finally,the HE and immunohistochemical staining of the tumor biopsy was performed.Results In the experimental group,the parameters of the tumor including BF,BV,pBV and ALP were significantly different between pre-and post treatment(P<0.05),whereas the MPI,PVP and HPI were not significant(P≥0.05).In the control one,all the parameters were not significant.The maximum diameters of the tumor were 0.828±0.319 cm and 0.573±0.435 cm before and after treatment in the experimental group(P=0.000),while those were 0.816±0.401 cm and 1.370±0.332 cm in the control one(P=0.005).And the MIP and ALP were positively correlated with VEGF and MVD respectively.Conclusion As a non-invasive alternative method,dual-source CT perfusion scans can be used to evaluate the hemodynamic changes of liver cancer with sorafenib treatment in rat model.更多还原