【摘要】 目的 探讨栓塞猪冠状动脉前降支制备急性心肌梗死动物模型的可行性及其技术分析。方法 健康成年雄性滇南小耳猪12头,麻醉后行股动脉穿刺,插管至冠状动脉行左、右冠状动脉造影术,采用自制可脱球囊永久植入冠状动脉前降支(left anterior descending branch,LAD)中远1/3段,术中行心电动态监护,并分别于栓塞术前、术后6 h、24 h、72 h及1周行心肌酶学、心肌坏死标记物检测,全部动物于1周后处死,行TTC染色及病理切片分析以评价模型建立是否成功。结果 11头猪饲养至实验终止,经心电图、相关实验室检测及病理染色证实模型制备成功,且显示梗死部位均集中在心尖段、左室前壁心尖段,部分模型累及室间隔心尖段。其中1头猪于术中发生室颤,抢救无效死亡。结论 运用自制可脱球囊栓塞LAD可成功建立猪急性心肌梗死模型,该方法模型制备安全性较高,梗塞部位集中,各项观察指标表现明确,病理结果肯定,是急性心肌梗死实验研究的理想动物模型。更多还原

【Abstract】 Objective Aimed to probe the feasibility and technology about the preparation of swine acute myocardial infarction model by embolizing the left anterior descending branch of coronary artery.Methods 12 healthy and mature male Diannan tiny-ear pigs were selected.Implanted self-made sacculus to their LAD permanently after femoral artery puncture and coronary angiography.In the course of operation,electrocardiograph monitoring was applied.Marker detections were performed pre-operation,post-operation 6h,24h,72h,and 1w respectively.All the selected pigs were killed after a week.Then the TTC staining and pathological section were applied to analyze and access the possibilities of building model.Results 11 pigs kept alive till the end of the experiments.It was proved that the preparation of model was successful through electrocardiograph,lab inspection and pathological staining.The result unveiled that the embolism positions were at cardiac apex and apica sections of left ventricular in all the tested pigs,interventricular septum was involved in some models.One pig dead owing to ventricular fibrillation during the operation.Conclusion It is possible to build swine acute myocardial infarction model using self-made sacculus.High safety,concentrated infarction areas,clear indicators and certain pathological results were acquired.It is a feasible animal model for studying acute myocardial infarction.更多还原