【摘要】 目的研究大黄牡丹汤组方(RPDP)对急性胰腺炎(Acute pancreatitis,AP)模型大鼠胰腺腺泡细胞外分泌功能和腺泡细胞内钙离子浓度(FI)的影响。方法 SD大鼠灌胃RPDP以制备大黄牡丹汤组方含药血清(RPDP-S);SD大鼠分为假手术组和AP模型组,分离胰腺腺泡细胞并与RPDP-S共同孵育,测定腺泡细胞淀粉酶分泌水平和FI变化。结果 AP模型大鼠腺泡细胞分泌淀粉酶水平较假手术组显著升高(P<0.05),经RPDP-S处理的AP模型大鼠腺泡细胞分泌淀粉酶水平显著降低(P<0.05);FI随AP模型病程延长而升高(P<0.05),RPDP-S可抑制AP模型大鼠腺泡细胞内FI升高(P<0.05)。结论 RPDP通能抑制AP大鼠胰腺腺泡细胞的外分泌功能,抑制腺泡细胞内钙离子的升高,降低腺泡细胞内FI超载。更多还原

【Abstract】 Objective To study the effect of Dahuang Mudan Decoction(RPDP) on exocrine function and intracellular Ca2 +concentration(FI) of pancreatic acinar cells in acute pancreatitis(AP) model rats.Method Dahuang Mudan Decoction was administrated to SD rats and the serum containing Dahuang Mudan Decoction(RPDP-S) wasprepared.SD rats randomly divided into three groups:the normal control group,the sham operation(SO) group and the AP group.Separate pancreatic acinar cells were isolated and co-incubated with RPDP-S,amylase secretion of pancreatic acinar cells and the change of FI were analyzed.Result Compared with the SO group,amylase secretion of pancreatic acinar cells was significantly increase in AP group(P < 0.05); and amylase secretion was significantly decrease in the pancreatic acinar cells treated with RPDP-S(P < 0.05),and the FI was raise up along with the prolonged disease(P < 0.05).Conclusion RPDP can decrease intracellular Ca2 +concentration,and inhibit the overload of FIand the exocrine function of pancreatic acinar cells.更多还原