【摘要】 目的检测淋巴瘤组织中4-1BBL的表达水平,并分析其临床意义。方法对80例淋巴瘤组织芯片,采用免疫组织化学的方法,采用不同抗体量化测定其CD20、4-1BBL N端和4-1BBL C端表达情况;并且应用Image-Pro Plus软件计算各病例的IOD值。结果霍奇金病(HD)患者中CD20抗原表达水平显著高于非霍奇金淋巴瘤(NHL)患者;N端和C端4-1BBL表达水平并无显著相关性,不同患者4-1BBL转变为s4-1BBL的比率不同;通过测定IOD值发现大多数s4-1BBL是通过MMP直接切割导致的;少部分s4-1BBL是直接表达出来转运到细胞外的。结论不同淋巴瘤患者4-1BBL的表达水平不同、S4-1BBL的产生机制也不同,针对不同患者应提供不同的免疫治疗策略。更多还原

【Abstract】 Objective To detect expression of 4-1BBL in lymphoma and its clinical significance. Methods Immunohistochemistry was performed in 80 cases of lymphoma tissue microarrays,and expression of CD20,C-terminus and N-terminus of 4-1BBL were detected by antibody quantification. Integrated optical density( IOD) was calculated using Image-Pro plus software. Results Expression of CD20 in Hodgkin’s disease was higher than that of Non-Hodgkin’s lymphoma. Expression of 4-1BBL between C-terminus and N-terminus had no significant correlations. The transformation ratio of s4-1BBL into 4-1BBL was different in different patients. IOD showed that a large proportion of s4-1BBL was derived from matrix metalloproteinase mechanism,and another small proportion of s4-1BBL was directly expressed and transported extracellular. Conclusion The expression of 4-1BBL and generation of s4-1BBL in different patients are different. Different immunotherapy strategies should be provided to different patients.更多还原