目的:研究乳腺癌患者血浆中的MiR-21表达水平,初步探讨其在乳腺癌中作为循环肿瘤细胞(circulating tumor cells,CTCs)的临床意义。方法:采用基于SYBR Green I染料荧光分析的茎环实时定量逆转录聚合酶链式反应(qRT-PCR)分别检测40例乳腺癌患者及15例正常对照者外周血中MiR-21的表达水平,了解MiR-21表达与临床病理指标的关系。结果:乳腺癌患者血浆中的MiR-21表达水平明显高于正常对照者(P<0.01)。MiR-21表达与患者月经状况、肿瘤大小、雌激素受体(ER)、孕激素受体(PR)和人类表皮生长因子受体2(HER-2)表达均无明显关系(P>0.05),与TNM分期、淋巴结转移相关(P<0.05)。结论:茎环qRT-PCR是一种快速、准确、定量检测MiR-21的方法,MiR-21是一个能有效辅助乳腺癌诊断和监测CTCs的潜在标志物。
【英文摘要】
Objective: To study the expression of MiR-21 in peripheral blood in patients with breast cancer,and explore its clinical significance for diagnosing and monitoring GC,and serve as a marker for the detection of CTCs in breast cancer patients. Methods: Peripheral blood samples were collected from 40 preoperative patients with breast cancer and 15 healthy volunteers. Steploop real- time reverse transcription- polymerase chain reaction( qRT- PCR)was used to detect the level of MiR- 21. Results: Patients with br...
| 
推荐 
点击下列相关研究机构和相关文献作者，可以直接查到这些机构和作者被《中国知识资源总库》收录的其它文献，使您全面了解该机构和该作者的研究动态和历史。
