【摘要】 目的:探讨CFTR对大鼠急性肺动脉栓塞时急性肺损伤肺脏水清除率的影响。方法:SPF级SD大鼠30只,建立大鼠左肺急性肺动脉阻塞模型。随机分为对照组,急性肺动脉梗阻4h和24h组,每组10只,分别测定左右肺的肺水容量,肺水清除率(AFC),应用CFTR激动剂和抑制剂进一步调节发生变化的肺水清除能力,real-time PCR方法测定CFTR蛋白mRNA在大鼠肺组织的表达。结果:急性肺动脉阻塞4h和24h组的左肺肺水清除率(AFC)明显升高,肺动脉阻塞24小时后,肺水容量上升。但保持血液灌流的右肺无明显变化。CFTR激动剂NS004调节阻塞的左肺,各组肺水清除率升高不明显,但是CFTR特异性抑制剂CFTRinh172可降低各组已经升高的肺水清除率。Real-time PCR证实4h和24h组的左肺CFTR mRNA水平轻度升高。结论:CFTR可能参与在大鼠急性肺动脉栓塞时肺水清除率的变化。更多还原

【Abstract】 Objective:Cystic fibrosis transmembrane conductance regulator(CFTR) is a kind of chlorine channel which could be regarded that it regulates infection.In this study,we investigated whether the CFTR is related with acute lung injury induced by acute artery embolism.Methods:There were 30 SD rats randomly divided into 3 groups:control,PAE 4h and PAE 24h.All were measured both lungs alveolar fluid clearance(AFC),lung weight wet-to-dry ratio,AFC affected by CFTR agonist and inhibitor,CFTR mRNA expression in lungs.Results:AFC of PAE 4h and 24h in embolism lung was increased and the lung weight wet-to-dry ratio was increased in PAE 24h group.The AFC and lung weight wet-to-dry of non-embolism lung was not changed in all groups.CFTR agonist-NS004 did not affected AFC in PAE 4h and 24h groups but CFTR-inh172 decreased the AFC in PAE 4h and 24h.Real-time PCR tested showed that CFTR mRNA obviously increased in PAE 4h and 24h group.Conclusion:CFTR may be modulate AFC in acute pulmonary embolism in mouse.更多还原