【摘要】 目的:对110例成人急性淋巴细胞白血病(ALL)患者进行免疫表型及细胞遗传学特征分析。方法:采用CD45/SSC参数设门四色流式细胞术检测110例成人ALL患者治疗前骨髓细胞的免疫表型,染色体R显带技术对其中的73例进行核型分析。结果:110例ALL患者中,21.8%为T-ALL,78.2%为B-ALL。47.3%的ALL患者表达髓系抗原(MyAg),CD13是成人ALL中最常见的MyAg(32.1%)。T-ALL髓系相关抗原表达总的阳性率(45.8%)与B-ALL(47.7%)无统计学差异。可供核型分析的73例中核型异常者37例(50.7%),最常见的遗传学异常为Ph染色体,占23.3%。结论:免疫表型对ALL的诊断与分型至关重要,免疫表型与患者的异常核型改变及临床特征关系密切。更多还原

【Abstract】 Objective: To study the immunophenotypic and cytogenetic features of adult acute lymphoblastic leukaemia( ALL) patients. Methods: All 110 adult patients with ALL were immunophenotyped by CD45 / SSC double parameters and four colour flow cytometry. Seventy- three cases were also subjected to karyotype analysis by R- banding technology. Results: Of the 110 adult patients with ALL,21. 8% were identified as T- ALL,78. 2% as B-ALL. Myeloid antigen( MyAg) expression was documented in 47. 3% of the 110 adult ALL cases analysed and CD13 was the most commonly expressed MyAg in ALL patients( 32. 1 %). No difference was observed in the expression of MyAg between the groups of patients with T- ALL( 45. 8%) and B- ALL( 47. 7%). Abnormal karyotypes were detected in 37 out of 73( 50. 7%) cases and the most common cytogenetic abnormality was the Philadelphia( Ph) chromosome,which was found in 23. 3% of the cases. Conclusion: Immunophenotype analysis was useful for ALL diagnosis and classification and the immunophenotype did have relevance to the abnormal cytogenetic changes and clinical features in ALL.更多还原