【摘要】 目的:研究p57kip2在人乳腺癌组织和细胞系中的表达和定位。方法:免疫印迹法检测人乳腺肿瘤细胞系中p57kip2的表达。人乳腺肿瘤组织进行免疫组化染色,检测p57kip2在组织中的表达。激光共聚焦扫描显微镜检测内源p57kip2在MCF-7细胞中的定位。结果:p57kip2在乳腺癌细胞系内表达,免疫组化染色分析和激光共聚焦扫描显微镜证实p57kip2主要定位于细胞核内。结论:p57kip2在乳腺癌细胞内表达,p57kip2主要定位于乳腺癌组织细胞核内,可能是参与肿瘤细胞基因转录调控和蛋白表达的重要蛋白之一。更多还原

【Abstract】 Objective: To investigate the expression and localization of p57kip2protein in human breast cancer cell lines. Methods: Western blot analysis was used to determine p57kip2protein expression in human breast cancer cell lines,immunohistochemistry analysis for exogenous p57kip2expression from human breast cnacer tissues. The endogenous localization of p57kip2in MCF- 7 cells was observed by using laser scanning confocal microscopy. Results:p57kip2protein was expressed in 3 different human breast cancer cell lines. The human p57kip2protein was localized mainly in the nucleus by immunohistochemistry,also confirmed by laser scanning confocal microscopy. Conclusion:p57kip2protein was expressed in breast cancer cell lines. p57kip2was localized mainly in the nucleus,which may be one of the mechanisms that attribute to signal transduction and protein expression of cancer cells.更多还原