【摘要】 目的:探讨高脂高糖饮食对日本大耳白兔糖代谢和脂代谢的影响,建立日本大耳白兔代谢综合征模型。方法:将20只雄性日本大耳白兔随机分为两组(每组10只),对照组饲喂基础饲料,实验组饲喂高糖高脂饲料(配方:10%猪油+30%蔗糖+60%基础饲料),观察36周,期间每2周抽血1次,检测血糖和甘油三酯的水平。于第36周结束时,对动物进行葡萄糖耐量试验,而后麻醉处死动物,观察动物重要脏器和全身脂肪重量变化。结果:实验组家兔在36周时,体重[(3.90±0.09)kg vs(3.18±0.27)kg]、脂肪重量[总脂肪:(493.40±55.25)g vs(331.45±38.28)g]和内脏脂肪[(354.90±48.18)g vs(214.35±23.74)g]均显著高于对照组,实验组和对照组之间比较存在显著性差异。结论:高糖高脂可诱导日本大耳白兔引起糖代谢紊乱和中心性肥胖,这为代谢综合征的研究提供实验和理论基础。更多还原

【Abstract】 Objective:To induce metabolic syndrome in Japanese white rabbit by high fat/high sucrose diet(HFSD).Methods:Twenty male Japanese white rabbits were divided into 2groups by randomized block design:normal diet group(n=10)and HFSD group(n=10,with 10%lard,30% white cane sugar and 60%normal fodder),and observed for 36weeks.The blood samples of the rabbits were tested for the levels of plasma glucose and triglyceride after overnight fasting every two weeks.At the end of 36th week,after intravenous glucose tolerance test(IVGTT),all the rabbits were killed to observe vital organs and weight all adipose tissues.Results:The difference between the 2groups was not significant in plasma glucose,triglyceride and insulin except in IVGTT.HFSD group were higher in body weight[(3.90±0.09)kg vs(3.18±0.27)kg]and adipose tissue[total adipose tissue:(493.40±55.25)g vs(331.45±38.28)g;visceral adipose tissue:(354.90±48.18)g vs(214.35±23.74)g]as compared with control group,and the difference was significant.Conclusion:HFSD can induce impaired glucose tolerance and trend of central obesity in Japanese white rabbits.更多还原