【摘要】 目的 监测2012年河北省细菌耐药性监测网(hebei antibacterial resistance investigation net,Hebarin)各成员单位临床分离细菌的菌种分布及对常用抗菌药物的耐药性谱型。方法 回顾性分析河北省2012年收集细菌药敏试验监测数据,采用K-B纸片法或微量肉汤稀释法药敏试验,判断标准按CLSI 2010年版,数据分析采用WHONET 5.5软件。结果2012年共收集细菌10 504株,其中革兰阴性菌8 003株,占76.2%;革兰阳性菌2 501株,占23.8%。革兰阴性杆菌中前五位的细菌分别为大肠埃希菌、肺炎克雷伯菌、铜绿假单胞菌、鲍曼不动杆菌和阴沟肠杆菌;革兰阳性球菌中前五位的细菌分别为金黄色葡萄球菌、屎肠球菌、粪肠球菌、肺炎链球菌和表皮葡萄球菌。大肠埃希菌和肺炎克雷伯菌中ESBL的检出率分别为66.5%和46.7%;大肠埃希菌、肺炎克雷伯菌和阴沟肠杆菌中对亚胺培南的耐药率分别为0.1%,0.5%和8.9%,对美罗培南的耐药率分别为0.1%,0.6%和4.2%。铜绿假单胞菌对亚胺培南和美罗培南的耐药率分别为38.9%和32.3%,而鲍曼不动杆菌对亚胺培南和美罗培南的耐药率分别为56.5%和59.7%。金黄色葡萄球菌和凝固酶阴性葡萄球菌中甲氧西林耐药株所占比率为57.5%和87.3%。未发现对利奈唑胺、万古霉素耐药的葡萄球菌属细菌,但是出现对替考拉宁耐药的凝固酶阴性葡萄球菌。结论 细菌耐药性监测对准确掌握细菌对抗菌药物的耐药动向和耐药性变迁,指导临床合理用药具有重要的意义。更多还原

【Abstract】 Objective To investigate antimicrobial resistance and pathogen in hebei antibacterial resistance investigation net in2012.Methods Antimicrobial susceptibility test was detected by Kirby-Bauer method or broth dilution test.Results were analyzed according to CLSI 2010 breakpoints.WHONET 5.5 software was used to analyze the data.Results A total of 10504 clinical isolates were collected in 2012,of which gram negative bacilli and gram positive cocci accounted for 76.2%,23.8%,respectively.The most common pathogen in gram-negative rod was E.coli,K.pneumoniae,P.aeruginosa,A.baumanii and E.cloacae respectively.The most common pathogen in gram-positive cocci was S.aureus,E.facium,E.faecalis,S.pneumoniae and S.epidermidis.ESBL rate of E.coli and K.pneumoniae was 66.5 and 46.7%.The resistant rate of E.coli,K.pneumoniae,E.cloacae to imipenem was 0.1%.0.5%,8.9%and to meropenem was 0.1%,0.6%,4.2%,respectively.P.aeruginosa was resistant to imipenem and meropenem were 38.9%and 32.3%.A.baumanii was resistant to imipenem and meropenem were 56.5%and 59.7%.Methicillin-resistant strains accounted for an average of 57.5%in S.aureus and 87.3%in coagulase negative staphylococcus.Staphylococcus was still susceptible to minocycline and chloramphenicol.No staphylococcal strains were found resistant to vancomycin,linezolid.But a few coagulase negative staphylococcal strains were resistant to teicoplanin.Conclusion Surveillance of antimicrobial agents played an important role in controlling hospital infection.更多还原