【摘要】 目的 检测窒息新生儿血清miR-21,miR-210和miR-424的表达水平,为microRNA在新生儿窒息的早期诊断和治疗提供理论依据。方法 运用RT-PCR方法检测62例窒息新生儿血清microRNA的表达水平。根据出生时Apgar评分,分为轻度窒息组45例,重度窒息组17例和30例健康孕妇脐血,以U6snRNA为内参。检测miR-21,miR-210和miR-424在血清中的表达水平。结果 3种microRNA在所有组中均可检测到,与对照组比较,miR-21和miR-210在窒息惠儿血清中表达水平上调4.57±0.41和4.18±0.32倍(t=3.02,2.72,P<0.05),且重度窒息组高于轻度窒息组(t=2.23,2.25,P<0.05)。miR-424表达水平无差异(1.34±0.19,t=0.29,P>0.05)。结论 miR-21和miR-210在窒息惠儿血清中表达上调,与病情严重性正相关,检测其在血清中的表达量对新生儿窒息的早期诊断与预后判断具有重要的临床意义。更多还原

【Abstract】 Objective To detect the expressions of serum miR-21,miR-210 and miR-424 in newborns with asphyxia and to find out the potentinal candidate mieroRNA for diagnosis and treatmen of asphyxia.Methods The expression relevant microRNAs in serum was detected using real-time fluorescent quantitative PCR(stem-loop RT-qPCR,Taqman probe).Level of serum miR-21,miR-210 and miR-424 of 62 cases newborns with neonatal asphyxia and 30 healthy were detected.The neo natal with asphyxia were divided into two groups,the severe asphyxia group(17 eases)and mild asphyxia group(45 cases).Results The level of miR-21,miR-210 and miR-424 were detected in all groups,but the level of miR-21 and miR-210 in severe group were significantly higher than the mild group(t=2.23,2.25,P<0.05)and the control group(4.57±0.41,4.18±0.32,t=3.02,2.72,P<0.05)。The level of miR-424 had no significant different(1.34:k0.19,t=0.29,P>0.05).Conclusion Detection of these microRNA in serum of patients can contribute to the clinical diagnosis,treatment and prognosis evaluation of neonatal asphyxia.更多还原