【摘要】 以D113离子交换树脂—海藻酸钠混合凝胶为载体,2%CaCl2溶液为固化液,采用包埋法共固定化糖化酶和酵母菌,并用于生料液态法白酒生产中。通过研究影响共固定化颗粒糖化发酵性能的因素,确定了共固定化的最佳条件:载体总量40mL,树脂浓度1.0%、海藻酸钠浓度3.5%、二氧化硅添加量为0.8%,糖化酶包埋量150mg,酵母菌包埋量60mg。将共固定化颗粒接入25%高粱粉发酵培养基中,30℃糖化发酵7d,酒度为15.0%。共固定化颗粒重复使用10次后,活力保存率为66.7%。更多还原

【Abstract】 In this study,glucoamylase and yeast were co-immobilized onto the composite gel of resin D113and sodium alginate by embedding method,with 2% CaCl2solution as the solidified liquid.By analyzing the main factors in saccharifying fermentability,the optimal condition of co-immobilization were 1.0% resin,3.5% sodium alginate,0.8% silicon dioxide,150mg glucoamylase,and 60mg yeast,with the final volume of 40ml.When co-immobilized glucoamylase and yeast were added into the fermentation medium with 25%sorghum powder for 7days at 30℃,the content of alcohol reached 15.0%,and the surplus vitality remained 66.7%,after 10times usage.更多还原