【摘要】 目的:以新型隐球菌荚膜相关蛋白CAP10为靶抗原,制备并鉴定特异性抗CAP10的单克隆抗体。方法:用纯化的重组CAP10免疫BALB/c小鼠,血清抗体效价达到适当水平时进行细胞融合;经多次亚克隆筛选出分泌特异性抗体的细胞株,制备单抗腹水并进行抗体效价测定及亚类鉴定。结果:获得11株能稳定分泌抗新型隐球菌荚膜相关蛋白CAP10的单克隆抗体杂交瘤细胞株,抗体效价高且抗原特异性强。结论:获得了针对新型隐球菌荚膜相关蛋白CAP10的单克隆抗体,为深入研究CAP10蛋白的功能,以及临床新型隐球菌的检测和血清型分析奠定了基础。更多还原

【Abstract】 Objective: To produce monoclonal antibodies(mAb) specific to CAP10 protein of Cryptococcus neoformans. Methods: The BALB/c mice were immunized with the recombinant CAP10-1(70~250 aa), and fusion oflymphocyte and myeloma Sp2/0 cell was performed by conventional method. Antibody titers and specificities ofmAb were measured by ELISA followed production of mAb. Results: Eleven strains of hybridoma cell lines withthe stable secretion of mAb to protein CAP10 were generated, and all the mAb only reacted with the recombinantCAP10-1. Conclusion: High titers of the specific mAb to the conserved fragment of CAP10 of C.neoformans weresuccessfully produced, which is helpful for further study of the function of CAP10 and clinical diagnosis and sero-type analysis for infections of C.neoformans.更多还原