【摘要】 本文采用单因素和正交试验设计,对链霉菌Streptomyces canus sp.FIM-0916产安福霉素的发酵培养基配方及发酵条件进行了优化。优化后的最佳培养基配方为:蔗糖1.5%,黄豆粉1.0%,组氨酸0.1%,KNO30.1%,CaCO30.1%。最佳发酵条件为:种子菌龄54 h,装液量120 mL/500 mL,接种量2%,发酵温度28℃,摇床转速250 r/min;最佳发酵时间为5 d。在该优化条件下,安福霉素的发酵效价比对照提高248%,为安福霉素的后续开发奠定了基础。更多还原

【Abstract】 The fermentation medium and conditions of Streptomyces canus sp. FIM0916 were optimizied for producing amphomycin by single-factor and orthogonal test design. The optimized medium formula is 1. 5% sucrose,3. 5% soybean meal,0. 1% L-histidine,0. 1% KNO3and 0. 1% CaCO3; the optimal fermentation conditions are as follows: filling volume is 120 mL for a 500 mL shake flask,shaker’s rotating speed is 250 r / min,inoculum size is 2%,temperature 28℃,and cultivation time of seed 54 h; the optimal fermentation time are 5 d. Under optimized conditions,the titer of amphomycin was 248% higher than that of control,which laid the foundation for subsequent development on amphomycin.更多还原