旨在对Streptomyces fradiae FIM-S38胞外产生的次级代谢产物新霉素进行发酵条件优化。采用单因素筛选结合正交试验优化发酵培养基,探讨摇瓶发酵的主要影响因子初始p H值、装液量、转速等发酵参数的影响,确定了适宜发酵培养基组成和较佳发酵参数:葡萄糖6.0%,可溶性淀粉3.5%,黄豆粉2.0%,硫酸镁0.1%,碳酸钙0.3%,初始p H7.0,装液量50 m L/500 m L,摇床转速250 r/min,发酵温度35℃,发酵时间6 d,优化后的发酵水平较原生产工艺提高了50%以上。
【英文摘要】
It was to optimize the fermentation conditions of neomycin producing by Streptomyces fradiae FIM-S38. The single factor experiments and orthogonal experiments were adopted to optimize the fermentation medium and conditions of FIM-S38 in shake flasks. The constituents of medium, the filling volume of shaking flask, the rotation speed and the temperature were the major factors that affected the yield of neomycin. The composition of glucose 6.0%, soluble starch 3.5%, soybean meal 2.0%, Mg SO4 0.1% and Ca CO3 0...
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