【摘要】 目的:采用高效液相色谱法(HPLC)测定抗宫炎凝胶剂中金石蚕苷的含量,为制定其质量标准提供基础。方法:采用十八烷基硅烷键合胶为填充剂,乙腈-0.5%磷酸(18:82)为流动相;检测波长为332 nm;流速为1.0 ml/min,探讨HPLC测定抗宫炎凝胶剂中金石蚕苷含量的专属性、精密度、稳定性、重现性等。结果:金石蚕苷在0.104~0.468 mg/ml范围内呈良好的线性关系(r=0.9999),平均回收率为99.76%,RSD为1.56%。结论:采用HPLC测定抗宫炎凝胶剂中金石蚕苷含量的专属性、精密度、稳定性、重现性均良好,该方法简便可靠,可用于抗宫炎凝胶剂的质量标准的研究。更多还原

【Abstract】 Objective: To establish a HPLC method to determine the contents of Poliumoside in the Kanggongyan Gels and provide foundation for the development of its quality standard. Methods: C18 was used as chromatographic column, the mobile phase was Acetonitrile-0.5%H3PO4(18:82), the wave lenghth was 332 nm. and the flow rate was 1.0ml/min. The specificity, precision, stability,and reproducibility of HPLC in the detection of content of Poliumoside in the Kanggongyan Gels were analyzed. Results: The Poliumoside showed good linear relationship in the range of 0.104 ~0.468 mg/ml and the average recovery was 99.76% with RSD of1.56%. Conclusion: The specificity, precision, stability, reproducibility of HPLC in the detection of content of Poliumoside in the Kanggongyan Gels were good. HPLC was a simple and reliable method for the research of quality standard of kanggongyan gels.更多还原