【摘要】 目的:观察组蛋白去乙酰化酶抑制剂(HDACIs)MS-275联合抗生素类化疗药物平阳霉素(PYM)对口腔鳞状癌细胞Tca-8113的生长及凋亡的影响。方法:以体外培养的口腔鳞状细胞癌Tca-8113细胞为研究对象,应用MTT法检测不同浓度(0、1、2、4、8μmol/L)MS-275、(0、0.05、0.1、0.2、0.4μmol/L)平阳霉素(PYM)单独和联合用药对Tca-8113细胞增殖活性的影响;Annexin-V-FITC/PI双染流式细胞术定量检测细胞的凋亡情况。结果:不同浓度(1、2、4、8μmol/L)MS-275和(0.05、0.1、0.2、0.4μmol/L)PYM均可显著抑制Tca-8113细胞的增殖,并促进其凋亡,且呈浓度依赖性(P<0.05)。4μmol/L MS-275和0.2μmol/L PYM联合应用时,其抑制Tca-8113细胞增殖和促进其凋亡的作用均显著高于单独应用MS-275或PYM(P<0.05)。结论:MS-275能有效增强口腔鳞状细胞癌Tca-8113细胞对平阳霉素(PYM)的敏感性,体外实验中呈现较好的抗肿瘤效应。更多还原

【Abstract】 Objective: To investigate the influence of combined application of histone deacetylase inhibitors MS-275 and antibiotics, chemotherapy drugs Pingyangmycin on the growth and apoptosis of oral squamous carcinoma cell Tca-8113. Methods: Oral squamous cell carcinoma Tca-8113 cells cultured in vitro were used for the study, MTT was performed to examine the proliferative activity of Tca-8113 cells after separated or combined application of(0, 1, 2, 4, 8μmol/L) MS-275and(0, 0.05, 0.1, 0.2, 0.4 μmol/L)PYM; Annexin V-FITC/PI double dye, Flow cytometry was used to detect the effects of MS-275 plus PYM on the apoptosis of Tca-8113 cells. Results: Different concentrations(1, 2, 4, 8 μmol/L) of MS-275 or(0.05, 0.1, 0.2, 0.4 μmol/L) PYM could both inhibit the growth and induce the apoptosis of Tca-8113 cells at a concentration dependent manner(P<0.05). Compared with MS-275 or PYM alone, 4μmol/L MS-275 plus 0.2 μmol/L PYM could more significantly inhibit the proliferation of Tca-8113 cells and promote the apoptosis(P<0.05). Conclusion: MS-275 could effectively enhance the sensitivity of Tca-8113 cells to PYM, which showed good anti-tumour effect in vitro.更多还原