【摘要】 目的:探讨DJ-1基因siRNA对三阴性乳腺癌细胞体外侵袭和迁移能力的影响。方法:设计DJ-1基因的小分子干扰RNA(siRNA)片段,脂质体介导转染入三阴性乳腺癌细胞株MAD-MB-23l,转染分3个组:A组(空白对照control组)、B组(转染非特异性对照Scramble组)、C组(转染si DJ-1组)。应用Western blotting免疫印迹法检测转染前后DJ-1表达水平;运用细胞迁移和侵袭实验检测细胞迁移和侵袭能力的变化。结果:C组DJ-1蛋白的表达强度弱于A组和B组(t=9.831,P<0.05),而A组与B组比较,DJ-1蛋白表达水平则无明显差异(t=1.629,P>0.05)。细胞迁移实验中,A组细胞为(218.37±12.75);B组的细胞为(214.46±11.38);C组的细胞为(129.65±8.59),C组细胞明显少于A组和B组(t=10.927,9.984,P<0.05),而A组与B组之间,差异无统计学意义(t=0.512,P>0.05)。细胞侵袭实验中,A组细胞为(127.28±12.65);B组的细胞为(123.06±13.08);C组的细胞为(52.85±9.58),C组穿过人工基底膜的细胞明显少于A组和B组(t=7.927,8.643,P<0.05),而A组与B组之间,差异无统计学意义(t=0.627,P>0.05)。结论:DJ-1基因siRNA可抑制三阴性乳腺癌细胞侵袭和迁移。更多还原

【Abstract】 Objective: To investigute the effect of RNA interference induced silencing of DJ-1 gene on migration and invasion potential in triple-negative breast cancer cells. Methods: Human breast cancer cells MAD-MB-23l were divided into control group(A group),Scramble group(B group) and si DJ-1 group(C group). siRNA sequence targeting DJ-1 gene were designed and synthesized. The siRNA were transfected into triple-negative breast cancer cell by lipofectamine TM 2000 mediation. The alteration of DJ-1 protein expression was detected by western blotting, Invasion potential were evaluated by transwell invasion assay, Cell migrating ability was detected by transwell migration assay. Results: Transfection of DJ-1 siRNA significantly knocked down the DJ-1 protein expression, The migration and invasion of breast cancer cells declined after transfection with siRNA DJ-1, DJ-1 protein expression of C group was lower than that of A group and B group(t=9.831, P<0.05). There was no difference between A group and B group(t=1.629, P>0.05). In cell migration assay, the number of cells in C group(129.65±8.59) was less than that in A group(218.37±12.75) and B group(214.46± 11.38)(t=10.927, 9.984, P<0.05). There was no difference between A group and B group(t=0.512, P>0.05). In cell migration assay, the number of cells in C group(129.65±8.59) was less than that in A group(218.37±12.75) and B group(214.46±11.38)(t=10.927, 9.984, P<0.05). There was no difference between A group and B group(t=0.512, P>0.05). In invasion potential test, the number of cells in C group(52.85±9.58) was less than that in A group(127.28±12.65) and B group(123.06±13.08)(t=7.927, 8.643, P<0.05). There were no difference between A group and B group(t=0.627, P>0.05). Conclusions: The siRNA targeting DJ-1 gene can restrain the migration and invasion of triple-negative breast cancer cell.更多还原