【摘要】 采用草鱼呼肠孤病毒腹腔注射草鱼,通过定量RT-PCR检测了12个抗病毒免疫相关基因在鳃中不同时间点的表达模式,以了解鳃对内源性病毒的免疫应答。模式识别受体基因CiTLR3、CiTLR7、CiTLR22、CiRIG-I、CiMDA5、CiLGP2、CiNOD1和CiNOD2,以及干扰素基因CiIFN-I的表达在注射病毒后12h、24h、48h及72h基本都上调。IgM基因的表达仅在72h上调。接头分子CiMyD88和CiIPS-1基因的表达在早期下调(6h),然后逐渐上升。为了证实病毒感染的可靠性,通过RT-PCR检测了病毒VP4基因。结果表明草鱼鳃在抗病毒免疫方面发挥着重要作用。更多还原

【Abstract】 Gill plays an important physical barrier role in defending environmental microbes. How are immune responses to endogenous viruses in gill? In the present study, mRNA expressions of 12 antiviral immune-related genes were examined by quantitative real-time RT-PCR(qRT-PCR) in grass carp(Ctenopharyngodon idella) gill after grass carp reovirus(GCRV) challenge. The relative values of CiTLR3, CiTLR7, CiTLR22, CiRIG-I, CiMDA5, CiLGP2, CiNOD1, CiNOD2 and CiIFN-I were almost up-regulated at 12 h, 24 h, 48 h and 72 h. Additionally, the mRNA expression of CiIgM was triggered at 72 h. However, relative expressions of CiMyD88 and CiIPS-1 were down-regulated at 6h, and subsequently increased. To further verify the reliability of viral infection, VP4 gene(outer capsid protein of GCRV, segment 6) was checked by RT-PCR amplification. The results indicate that gill serves as an important immune organ, and plays crucial roles in triggering antiviral immune responses in grass carp.更多还原