【摘要】 试验以玉米麸皮为原料,采用平菇液体发酵法制备阿魏酰低聚糖和膳食纤维,同时测定发酵过程中淀粉酶、蛋白酶、纤维素酶和木聚糖酶的活性,并对发酵所得膳食纤维含量及持水性、膨胀力和持油力等性质进行了研究。结果表明玉米麸皮经过平菇发酵8 d后,发酵液中淀粉酶、蛋白酶、纤维素酶和木聚糖酶的酶活均达到最高,分别为978.67 mU/mL、10.15U/mL、256.50、100.76 mU/mL,发酵液中FOs含量为12.515μmol/L。不溶性膳食纤维的提取率为2.83±0.79 g/mL,其持水性、膨胀力和持油性分别为1.37±0.39 g/g、2.17±0.29 mL/g和6.29±0.42 g/g,而可溶性膳食纤维的提取率为0.21±0.01 g/mL,其持水性、膨胀力和持油性分别为4.42±0.01 g/g、1.00±0.07 mL/g和4.75±0.49 g/g。平菇发酵玉米麸皮生产阿魏酰低聚糖和膳食纤维是可行的。更多还原

【Abstract】 In this study, feruloyl oligosaccharides and dietary fiber were obtained by submerged fermentation of edible mushroom using maize bran as the material. The activity of amylase, protease and cellulase produced by Pleurotus ostreatus and Phellinus igniarius were determined respectivly during fermentation, and then the content, the water retention, expansibility and oil absorbency of dietary fiber were further studied. The results were as follows: The activity of amylase, protease and cellulase produced by Pleurotus ostreatus during corn bran fermentation all reached the maximum of 978.67 mU/mL, 10.15 U/mL and 256.50 mU/mL respectively and the yield of FOs reached 12.515 mmol/L after 8 d. The yield of insoluble dietary fiber produced by Pleurotus ostreatus was 2.83±0.79 g/mL, and its water retention, expansibility and oil absorbency was 1.37±0.39 g/g, 2.17±0.29 mL/g and 6.29±0.42 g/g respectivly.The yield of soluble dietary fiber by Pleurotus ostreatus was 0.21±0.01 g/mL, and its water retention expansibility and oil absorbency was 4.42±0.01 g/g, 1.00±0.07 mL/g and 4.75±0.49 g/g respectivly.Key words:更多还原