【摘要】 采用加热回流提取、超声提取和微波提取法对厚朴进行提取,对比研究了3种提取方法对总黄酮、总酚、厚朴酚、和厚朴酚的含量及体外抗氧化活性的影响。结果表明,3种方法中,微波提取法对总黄酮、总酚及和厚朴酚的提取率最高,加热回流法对厚朴酚的提取率最高。3种提取物均能有效地清除DPPH·和HO·自由基,清除能力依次为微波提取、回流提取和超声提取;微波法厚朴提取物清除DPPH·和HO·自由基的IC50值分别为0.013、0.144 mg/mL。更多还原

【Abstract】 Three different extraction methods which heat reflux, ultrasonic and microwave extraction methods were used to extract active compounds from Magnolia officinalis. The effect of active composition and antioxidant activity with the three methods were investigated. The results showed that the extraction yields of total flavone, total polyphenol and honokiol by microwave extraction were highest. The extraction yield of magnolol by heat reflux extraction was highest. The extract had good scavenging activity on DPPH· and HO·, and the scavenging activity order of three methods was microwave >heat reflux >ultrasonic. The IC50 value of DPPH· and HO· scavenging by microwave extract was 0.013, 0.144 mg/mL, respectively.更多还原