【摘要】 采用大孔树脂及中压液相色谱对蓝莓果渣酸乙醇提取液中花色苷进行纯化。结果表明:AB-8大孔树脂具有较好的吸附解吸特性,适合蓝莓果渣花色苷的初级纯化。最佳吸附条件为上样溶液浓度0.8 mg/mL,最佳吸附pH2.0,上样速率1.0 mL/min;最佳解吸条件为乙醇体积分数60%,解吸pH1.0,洗脱速率1.0 mL/min。经大孔树脂纯化,其纯度为38.49%。然后经中压液相色谱纯化,花色苷纯度提高到94.42%。此法简便、高效,能制得高纯度的花色苷。更多还原

【Abstract】 Anthocyanins from blueberry pomace ethanol extract were purified by macroporous resin and medium-pressure liquid chromatography. The results showed that the AB-8 macroporous resin has better adsorption and desorption characteristics, so it is most suitable for primary purification on anthocyanins from blueberry pomace. The optimum adsorption conditions of AB-8 macroporous resin were that anthocyanins concentration is 0.8 mg/mL, pH value is 2.0, the sample rate is 1.0 mL/min. The optimum desorption conditions were that ethanol volume fraction is 60%, pH value is 1.0, and elution rate is 1.0 mL/min. The purity of anthocyanins, purified by macroporous resin, is 38.49%. Then the anthocyanins were purified by medium-pressure liquid chromatography, and its purity increased to 94.42%. This method is simple, efficient, and capable of producing high purity anthocyanins.更多还原