【摘要】 建立了离子排斥色谱法(ion-exclusion chromatography,IEC)测定黄酒中有机酸含量的分析方法。使用Waters离子排斥色谱柱(300 mm×7.8 mm,7μm),流动相为H2SO4溶液(A)与乙腈(B)的混合溶液(体积比为98∶2),线性梯度程序:0~40 min,流动相A的浓度由0.01 mol/L上升到0.02 mol/L;40~50 min,流动相A的浓度为0.01 mol/L;流速为0.5 mL/min,柱温50℃,进样量10μL,检测波长210 nm。结果表明,该方法可在30 min内实现草酸、马来酸、柠檬酸、酒石酸、苹果酸、抗坏血酸、琥珀酸、乳酸、富马酸、乙酸、丙酸、异丁酸和丁酸的完全分离与定量,13种有机酸在0.001~1.000 g/L范围内线性关系良好,回归方程的线性相关系数在0.999 7以上。黄酒中13种有机酸的加标回收率为93.4%~103.8%,相对标准偏差为0.1%~1.5%(n=5)。该方法简单快捷、准确、重复性好,可用于黄酒中有机酸的测定。更多还原

【Abstract】 An ion-exclusion chromatographic method for the simultaneous determination of organic acids in rice wine was developed.An IC-Pak Ion Exclusion column(300 mm×7.8 mm,7μm)was used at 50℃.The mobile phases were H2SO4(p hase A)and acetonitrile(p hase B)(98∶2,v/v)at a flow rate of 0.5 mL/min.The gradient elution program was as follows:0-40 min,0.01 mol/L H2SO4to 0.02 mol/L H2SO4;40-50 min,0.01 mol/L H2SO4.The injection volume was 10μL.The detection wavelength was set at 210 nm.The results showed that oxalic acid,maleic acid,citric acid,tartaric acid,malic acid,ascorbic acid,succinic acid,lactic,fumaric acid,acetic acid,p ro p ionic acid,isobut y ric acid and butyric acid were completely separated and determined in 30 min.The linear correlation coefficients were above 0.999 7 in the range of 0.001-1.000 g/L.Under the optimized conditions,the recoveries of organic acids in rice wine were in the range of 93.4%-103.8% with the relative standard deviations(RSDs,n=5)of 0.1%-1.5%.This method is feasible,convenient,fast,accurate and applicable for the quantitative analysis of the organic acids in rice wine.更多还原