【摘要】 目的:研究天冬种子油的理化性质和营养成分含量,观察天冬种子油对小鼠的镇静催眠作用,并评价其急性毒性。方法:采用中国油脂标准方法测定天冬种子油基本营养成分和理化性质;气相色谱-质谱联用法分析不皂化物成分;原子吸收分光光度计(atomic absorption spectrometry,AAS)法检测矿质元素含量;以KM种小鼠为受试动物,研究天冬种子的镇静催眠作用,并考察其急性毒性。结果:天冬种子油水分含量为1.87 g/100 g、灰分含量为0.15 g/100 g、粗脂肪含量为96.6 g/100 g、VE含量为405.55μg/g;不皂化物主要为烷烃类化合物(17%)和甾醇类化合物(41.8%);天冬种子油中含量较高的矿质元素有钠(297.92μg/g)、镁(216.88μg/g)、钙(145μg/g);天冬种子油4.65 g/kg和2.33 g/kg剂量组对小鼠自主活动有明显抑制作用,且能显著增加阈下剂量戊巴比妥钠小鼠睡眠只数;一次性灌胃最大给药量40 mL/kg(37.24 g/kg),连续观察14 d,未见死亡或体质量、活动等异常。结论:天冬种子油含有丰富的营养成分,具有安神镇静的作用,并且安全性较高。更多还原

【Abstract】 Objective: The aims of this study were to research the physico-chemical properties and nutrient contents of Radix Asparagi seed oil, to observe the sedative-hypnotic effects in mice and to evaluate its acute toxicity. Methods: The physicochemical properties of Radix Asparagi seed oil were determined according to the Chinese national standard method. Its unsaponifiable matters were detected by gas chromatography-mass spectrometry(GC-MS). The contents of mineral elements were measured by atomic absorption spectrophotometry(AAS). KM mice were used to evaluat e its acute toxicity and to observe its effects on spontaneous motion and synergism with pentobarbital sodium at a subliminal dose in mice. Results: The contents of moisture, ash, crude fat and vitamin E of the seed oil were determined to be 1.87 g/100 g, 0.15 g/100 g, 96.6 g/100 g and 405.55 μg/g, respectively. The results of GC-MS analysis showed that the unsaponifiable matters mainly contained sterol compounds(41.8%) and hydrocarbon compound(17%), etc. A variety of minerals were detected, with sodium, magnesium and calcium being d ominant at levels of 297.92, 216.88 and 145 μg/g, respectively. When administered at doses of 4.65 and 2.33 g/kg, the oil had obvious inhibitory effect on spontaneous motion in mice, and greatly incr eased the number of sleeping mice induced by pentobarbital sodium at subliminal dose. During the 14-day observation period after a maximum single dose of the seed oil, no mice died and no abnormality in body weight or motion was observed. Conclusion:Radix Asparagi seed oil is rich in nutrients, has the sedative-hypnotic effect and is of high safety.更多还原