【摘要】 以河南南阳蜂胶为原料,采用反相高效液相色谱-二极管阵列检测器-电喷射串联质谱对蜂胶乙酸乙酯提取物进行分离鉴定。以10种黄酮对照品为分析对象建立分析方法,10种黄酮对照品的线性相关系数为0.998 2~0.999 9,加标回收率为93.40%~103.69%,且精密度、稳定性和加标回收率的相对标准偏差均小于5%。采用该法对蜂胶乙酸乙酯提取物进行分析,河南蜂胶中可能含有槲皮素、芹菜素、山奈酚、异鼠李素、白杨素、松属素、高良姜素、短叶松素、短叶松素-3-乙酸酯、短叶松素-3-丙酸酯和短叶松素-3-丁酸酯11种黄酮类化合物以及对甲氧基肉桂酸肉桂酯和咖啡酸苯乙酯2种酚酸及其酯类化合物。更多还原

【Abstract】 The purpose of this study is to separate and identify ethyl acetate extract of propoils from Nanyang, central China’s Henan province by reverse phase-high performance liquid chromatography-diode array detector compled with electro spracy ionization-tandem mass spectrometry(RP-HPLC-DAD-ESI-MS-MS). A method for detecting 10 flavonoid reference standards was established using RP-HPLC-DAD. The linear correlation coefficients for the ten flavonoids were 0.998 2–0.999 9 and the recoveries were 93.40%–103.69%. The RSDs of precision, stability, and spiked recovery were all less than 5%. The ethyl acetate extract of Henan propolis was analysed by RP-HPLC-DAD-ESI-MS-MS. Totally 11 flavonoids were identified including quercetin, apigenin, kaempferol, isorhamnetin, chrysin, pinocembrin, galangin, pinobanksin, pinobanksin-3-O-acetate, pinobanksin-3-O-propionate, pinobanksin-3-O-butyrate and two phenolic acids and their esters.更多还原