【摘要】 建立无需皂化-高效液相色谱法同时测定牛奶中脂溶性维生素A、D和E的方法。牛奶样品与乙腈充分混合后经震荡、离心、浓缩、定容后应用高效液相色谱法检测,采用外标法对其进行定量分析。结果表明:3种维生素在各自相应浓度范围内线性关系良好,相关系数均大于0.999 0方法的检出限和定量限分别为0.01μg·m L-1~0.03μg·m L-1和0.03μg·m L-1~0.08μg·m L-1,在低、中、高3个水平下的加标回收率在91.23%~97.45%范围内,相对标准偏差为1.45%~3.68%(n=6)。该方法前处理简单、无需皂化、定量结果准确可靠,可以应用于乳品中脂溶性维生素的同时检测。更多还原

【Abstract】 To establish a without saponification-HPLC method for the determination of vitamin A, vitamin D and vitamin E in milks. Milk sample were extracted with acetonitrile, followed by shaking, centrifuging, concentration and redissolving. The chromatographic analysis was performed by HPLC and then external standard method was applied to quantification. The results indicated that three fat-soluble vitamins standards had a good linearity(r>0.999 0). The detection limit ranged was 0.01 μg·m L-1~0.03 μg·m L-1 and the quantitation limit range was 0.03 μg·m L-1~0.08 μg·m L-1. The mean recoveries at three spiked levels were between 91.23% and 97.45% with relative standard deviation(RSD) of 1.45%~3.68%(n=6). This method is simple, without saponification and accurate, which is appropriate for the determination of fat- soluble vitamins for milk.更多还原